![ENIGMA diversity](https://enigma.lbl.gov/wp-content/uploads/sites/12/2021/06/ENIGMA-diversity.jpg)
Lab-based ENIGMA researchers enrich, isolate and physiologically/genetically characterize the specific taxa that are predicted to be the most important to the observed field processes. By characterizing genetic functions and linking genotype to phenotype in the laboratory, we can interpret field data mechanistically, leading to pointed hypotheses of the role of particular taxa, proteins, co-factors, and their interactions in creating systems behavior.
![](https://enigma.lbl.gov/wp-content/uploads/sites/12/2024/03/ENIGMA_Bioepic1-525x350.jpg)
Relevant Publications
Multi-disciplinary research develops a deeper understanding of subsurface microbiology - ENIGMA is organized into three aims that reflect the different research scales (i.e., field level to molecular level) required to build a comprehensive picture of subsurface microbiology at the Oak Ridge Reservation (ORR): Subsurface Observatory, Environmental Atlas, and Environmental Simulations. Several key research initiatives, described in this cross-aim overview, highlight this framework in action. More →
![Schematic of upp deletion method.](https://enigma.lbl.gov/wp-content/uploads/sites/12/2023/06/Schematic-of-upp-deletion-method-150x150.png)
![](https://enigma.lbl.gov/wp-content/uploads/sites/12/2023/11/PhageMulticoloredBubbles-150x150.jpg)
![Schematic diagram showing the process of inputting cells of synthetic community and environment isolates into an automated benchtop liquid handler to prepare them for high-throughput proteomic analysis.](https://enigma.lbl.gov/wp-content/uploads/sites/12/2023/10/Cell-lysis-protocol-diagram-150x150.png)
![](https://enigma.lbl.gov/wp-content/uploads/sites/12/2023/09/EESA_Microbes-150x150.jpg)
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