SCIENCE: A key ENIGMA Aim is linking field data to the phenotypic characterization of isolates, testing a mechanistic hypothesis in the laboratory.
EISA-MRM (Enhanced In-Source Fragmentation/Annotation Multiple Reaction Monitoring):
This novel quantitative mass spectrometry technique allows the measurement of concentrations of the metabolites in ENIGMA samples using much cheaper instrumentation. These efforts characterizing key metabolites developing experimental quantitative methods to monitor the change of metabolome in bacteria are mission critical. The techniques will be used to design and test field-derived hypothesis in the laboratory.
ENIGMA Collaborators: Adam Deutschbauer Lab; Mike Adams Lab
$1 column cost (assuming 500 runs/column)
$1 electricity and solvents
$1 personnel time (assuming user is multi-tasking)
$2 instrumentation time (~60,000 hours instrument lifetime)
$5 quantitative MS metabolome