Berkeley Lab

Parts-Based Strategy Speeds Up Gene Function Annotation

Hualan Liu an ENIGMA Researcher in Adam Deutschbauer‘s Lab at Lawrence Berkeley Lab announces a powerful genetic tool for gene function annotation, essential to advance our understanding of microbial biology and the impact of those genetic traits on their ecosystems. 

She says “We developed a parts-based strategy for constructing and evaluating hundreds of transposon delivery vectors in parallel, and then select an effective one to generate large-scale mutant libraries in diverse bacteria. We successfully applied this strategy to 5 genera of bacteria, including two from the Oak Ridge National Lab field site.”


Liu, H.; A.M. Deutschbauer (2018) Rapidly moving new bacteria to model-organism status. Current Opinion in Biotechnology. DOI:10.1016/j.copbio.2017.12.006