Berkeley Lab

Nitrite Reduction Assay for Whole Pseudomonas Cells

ENIGMA researchers  at University of Georgia developed and published a rapid high-throughput assay for whole cell nitrite reductase activity.  Such activity assays will be key for future ENIGMA endeavors to relate biological activities of individual microorganisms and their communities to the environment.

  • A rapid high-throughput assay was constructed to measure whole cell nitrite reductase activity.
  • This detailed procedure can be adapted to investigate nitrate and nitrite reduction in ENIGMA relevant strains and communities.

Standard nitrite curve from 0-100 µM nitrite A) The red-pink color develops when Griess reagent is added to the nitrite containing sample. B) A linear standard curve is shown with values ranging from 0–100 µM nitrite

Nitrite Reduction Assay for Whole Pseudomonas Cells. Thorgersen, M.P.; and M.W. Adams Bio-protocol 6(10): e1818. http://www.bio-protocol.org/e1818