Berkeley Lab

Stable protein interactome of the model sulfate reducing bacterium Desulfovibrio vulgaris Hildenborough

First demonstration of a highly accurate, genome-wide method for detecting stable protein-protein interactions, without the requirement for genetic manipulation of the organism (prior tagless methodologies have false discovery rates of >85%). Combined with our study of protein complexes published in February 2016, which was based on affinity purification coupled with mass spectroscopy (AP-MS), this experiment […]

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Benchmark properties for highly accurate bacterial protein interactions

SUMMARY Numerous affinity purification – mass-spectrometry (AP-MS) and yeast two hybrid (Y2H) screens have each defined thousands of pairwise protein-protein interactions (PPIs), most between functionally unrelated proteins. The accuracy of these networks, however, is under debate. Here we present an AP-MS survey of the bacterium Desulfovibrio vulgaris together with a critical reanalysis of nine published […]

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SIFTER: Statistical Inference of Function Through Evolutionary Relationships

SIFTER Website →

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